| 產(chǎn)品編號 |
bs-13702R-PE-Cy3 |
| 英文名稱(chēng) |
Rabbit Anti-SKAP55/PE-Cy3 Conjugated antibody
|
| 中文名稱(chēng) |
PE-Cy3標記的SKAP55蛋白抗體 |
| 別 名 |
pp55;
SCAP 1;
SCAP1;
SKAP 1;
SKAP 55;
SKAP-55;
Skap1;
SKAP1_HUMAN;
SKAP55 adaptor protein;
SRC family associated phosphoprotein 1;
Src family-associated phosphoprotein 1;
Src kinase associated phosphoprotein 1;
SRC kinase associated phosphoprotein 55 kD;
Src kinase associated phosphoprotein of 55 kDa;
Src kinase-associated phosphoprotein 1;
Src kinase-associated phosphoprotein of 55 kDa. |
| 規格價(jià)格 |
100ul/2980元
購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) |
100ul
|
| 研究領(lǐng)域 |
細胞生物 免疫學(xué) 信號轉導 跨膜蛋白 |
| 抗體來(lái)源 |
Rabbit |
| 克隆類(lèi)型 |
Polyclonal |
| 交叉反應 |
(predicted: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, )
|
| 產(chǎn)品應用 |
ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 |
41kDa |
| 性 狀 |
Lyophilized or Liquid |
| 濃 度 |
1mg/ml |
| 免 疫 原 |
KLH conjugated synthetic peptide derived from human SKAP55 |
| 亞 型 |
IgG |
| 純化方法 |
affinity purified by Protein A |
| 儲 存 液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background:
Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines. SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.
Function:
Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Subcellular Location:
Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
Tissue Specificity:
Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
Post-translational modifications:
Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
Similarity:
Belongs to the SKAP family.
Contains 1 PH domain.
Contains 1 SH3 domain.
Database links:
Entrez Gene: 8631 Human
Entrez Gene: 286975 Rat
Omim: 604969 Human
SwissProt: Q86WV1 Human
SwissProt: Q4V7G1 Rat
Unigene: 316931 Human
Unigene: 35285 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
|
|
|
