| 產(chǎn)品編號 | bs-12343R-PE |
| 英文名稱(chēng) | Rabbit Anti-TRIB1/PE Conjugated antibody |
| 中文名稱(chēng) | PE標記的G蛋白偶聯(lián)誘導蛋白2受體抗體 |
| 別 名 | Tribbles homolog 1; TRB-1; G-protein-coupled receptor-induced gene 2 protein; GIG-2; SKIP1; TRIB1_HUMAN; C8FW; GIG2; TRB1; Trib1; Tribbles homolog 1. |
| 規格價(jià)格 | 100ul/2980元 購買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul |
| 研究領(lǐng)域 | 發(fā)育生物學(xué) 信號轉導 干細胞 細胞凋亡 激酶和磷酸酶 細胞分化 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Monkey, ) |
| 產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 41kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human TRIB1/GIG2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: TRB-1 is a 372 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase superfamily. Expressed ubiquitously with highest expression in bone marrow, thyroid gland, skeletal muscle and pancreas, TRB-1 interacts with MAPK kinases and is thought to regulate the activation of MAP kinses, possibly controlling MAP kinase cascades. The gene encoding TRB-1 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Function: Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity. Subcellular Location: cytoplasm. Tissue Specificity: Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 10221 Human Entrez Gene: 211770 Mouse Omim: 609461 Human SwissProt: Q96RU8 Human SwissProt: Q8K4K4 Mouse Unigene: 444947 Human Unigene: 40298 Mouse Unigene: 204391 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Trib1在脂肪組織維持和脂質(zhì)代謝紊亂上發(fā)揮關(guān)鍵作用; 巨噬細胞至少有兩個(gè)亞類(lèi)，即M1型和M2型。M1型巨噬細胞參與促炎反應，且在宿主防御細菌和病毒感染中發(fā)揮核心作用。M2巨噬細胞與抗炎反應、寄生蟲(chóng)感染、組織重構、纖維化以及腫瘤疾病發(fā)展相關(guān)。 Trib1是個(gè)銜接蛋白，與泛素連接酶COP1相互作用參與蛋白降解。人類(lèi)全基因組關(guān)聯(lián)研究表明TRIB1參與脂質(zhì)代謝。 日前，大阪大學(xué)WPI免疫前沿研究中心等處的研究人員，發(fā)現Trib1對F4/80 + MR +組織內在巨噬細胞的分化至關(guān)重要，且這些巨噬細胞和M2巨噬細胞、酸性粒細胞具有共同的特性(稱(chēng)之為M2類(lèi)巨噬細胞)。但是，Trib1在M1髓系細胞就不會(huì )發(fā)揮這種作用。相關(guān)研究成果刊登在近期出版的《自然》（Nature）雜志上。 Trib1缺陷導致各種器官中M2類(lèi)巨噬細胞急劇減少，如骨髓、脾臟、肺以及脂肪組織。在Trib1缺失的骨髓細胞中，C / EBP α的異常表達要為巨噬細胞分化缺陷負責。沒(méi)有想到的是，造血細胞缺乏 Trib1的小鼠，減少了脂肪組織塊，并伴隨著(zhù)脂肪分解增加，即使是在正常飲食條件下。 M2類(lèi)巨噬細胞的補充可緩解這種病理學(xué)現象。這表明，這類(lèi)巨噬細胞的缺乏是引發(fā)脂肪分解的原因。為了應對高脂肪飲食，造血細胞缺乏 Trib1的小鼠發(fā)育中會(huì )呈現高甘油三酯血癥、胰島素抗性，同時(shí)增加促炎細胞基因誘導。 這些結果表明Trib1在脂肪組織維持和脂質(zhì)代謝紊亂上發(fā)揮關(guān)鍵作用，而其作用是通過(guò)控制組織內在M2類(lèi)巨噬細胞的分化實(shí)現的。 |
