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    CD81 Mouse mAb (bsm-51378M)  
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    產(chǎn)品編號 bsm-51378M
    英文名稱(chēng) CD81 Mouse mAb
    中文名稱(chēng) CD81小鼠單克隆抗體
    別    名 26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan-28; Tspan28.  
    研究領(lǐng)域 細胞生物  免疫學(xué)  信號轉導  淋巴細胞  b-淋巴細胞  
    抗體來(lái)源 Mouse
    克隆類(lèi)型 Monoclonal
    克 隆 號 8C1
    交叉反應 Human
    產(chǎn)品應用 WB=1:500-5000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 26 kDa
    檢測分子量
    細胞定位 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD81 
    亞    型 IgG1
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產(chǎn)品介紹 The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008]

    Function:
    May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.

    Subunit:
    Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Interacts with CD53 and SCIMP.

    Subcellular Location:
    Membrane.

    Tissue Specificity:
    Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

    DISEASE:
    Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

    Similarity:
    Belongs to the tetraspanin (TM4SF) family.

    SWISS:
    P60033

    Gene ID:
    975

    Database links:

    Entrez Gene: 975 Human

    Omim: 186845 Human

    SwissProt: P60033 Human

    Unigene: 54457 Human



    產(chǎn)品圖片
    25 ug total protein per lane of various lysates (see on figure) probed with CD81 monoclonal antibody, unconjugated (bsm-51378M) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    Blank control(black line):HepG2. Primary Antibody (green line): Mouse Anti-CD81 antibody (bsm-51378M) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-mouse IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line): Normal Mouse IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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