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    CD80 Mouse mAb (bsm-60904M)  
    訂購熱線(xiàn):400-901-9800
    訂購郵箱:sales@bioss.com.cn
    訂購QQ:  400-901-9800
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    50ul/1580.00元
    100ul/2500.00元
    大包裝/詢(xún)價(jià)

    產(chǎn)品編號 bsm-60904M
    英文名稱(chēng) CD80 Mouse mAb
    中文名稱(chēng) 刺激分子B7-1蛋白單克隆抗體
    別    名 Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80.  
    抗體來(lái)源 Mouse
    克隆類(lèi)型 Monoclonal
    克 隆 號
    交叉反應 Human
    產(chǎn)品應用 IHC-P=1:20-100,IHC-F=1:20-100,IF=1:20-100
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 32 kDa
    檢測分子量
    細胞定位 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD80 
    亞    型 IgG1, k
    純化方法 affinity purified by Protein G
    緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產(chǎn)品介紹 CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

    Function:
    Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

    Subcellular Location:
    Membrane; Single-pass type I membrane protein.

    Tissue Specificity:
    Expressed on activated B-cells, macrophages and dendritic cells.

    Similarity:
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    SWISS:
    P33681

    Gene ID:
    941

    Database links:

    Entrez Gene: 941 Human

    Entrez Gene: 12519 Mouse

    Omim: 112203 Human

    SwissProt: P33681 Human

    SwissProt: Q00609 Mouse

    Unigene: 838 Human

    Unigene: 89474 Mouse



    產(chǎn)品圖片
    Paraformaldehyde-fixed, paraffin embedded (human thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD80) Monoclonal Antibody, Unconjugated (bsm-60904M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD80) Monoclonal Antibody, Unconjugated (bsm-60904M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD80) Monoclonal Antibody, Unconjugated (bsm-60904M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD80) Monoclonal Antibody, Unconjugated (bsm-60904M) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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