| 產(chǎn)品編號 | bsm-52254R |
| 英文名稱(chēng) | GFAP Recombinant Rabbit mAb |
| 中文名稱(chēng) | 膠質(zhì)纖維酸性蛋白重組兔單抗 |
| 別 名 | Astrocyte; FLJ45472; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN. |
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Specific References (1) | bsm-52254R has been referenced in 1 publications.
[IF=4.755] Hou Yujun. et al. Maintenance of Intestinal Homeostasis in Diarrhea-Predominant Irritable Bowel Syndrome by Electroacupuncture Through Submucosal Enteric Glial Cell-Derived S-Nitrosoglutathione. FRONT PHYSIOL. 2022 Aug;0:1368 IF ; Rat.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉導 干細胞 細胞粘附分子 細胞類(lèi)型標志物 細胞骨架 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號 | 3F4 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:20000,IHC-P=1:100-1:500,IHC-F=1:100-1:500,IF=1:100-1:500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GFAP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] Function: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Subunit: Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus). Subcellular Location: Cytoplasm. Note=Associated with intermediate filaments. Tissue Specificity: Expressed in cells lacking fibronectin. Post-translational modifications: Phosphorylated by PKN1. DISEASE: Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Similarity: Belongs to the intermediate filament family. SWISS: P14136 Gene ID: 2670 星形膠質(zhì)細胞標志物 （Astrocyte Marker） GFAP是一個(gè)56kDa的中間絲蛋白（intermediate filament，IF），在中樞神經(jīng)系統發(fā)育期是一個(gè)特異性的標志物，以區別星形細胞和其它膠質(zhì)細胞。GFAP表達在皮層和海馬,急、慢性皮質(zhì)酮治療時(shí)表達減少。 GFAP可以和人、大鼠、小鼠的GFAP反應，在正常和腫瘤性的星形膠質(zhì)細胞陽(yáng)性表達，而神經(jīng)節細胞、神經(jīng)元、成纖維細胞、少突膠質(zhì)細胞和這些細胞來(lái)源的腫瘤細胞陰性表達，主要用于星形膠質(zhì)瘤等中樞神經(jīng)系統腫瘤的診斷和鑒別診斷,GFAP的缺乏可導致AD病。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (bsm-52254R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP? Monoclonal Antibody, Unconjugated(bsm-52254R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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