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    BCA Protein Assay Kit (C05-02001)  
    訂購熱線(xiàn):400-901-9800
    訂購郵箱:sales@bioss.com.cn
    訂購QQ:  400-901-9800
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    40ml(200T)/180.00元
    100ml(500T)/318.00元
    100ml×10(5000T)/2400.00元
    大包裝/詢(xún)價(jià)

    產(chǎn)品編號 C05-02001
    英文名稱(chēng) BCA Protein Assay Kit
    中文名稱(chēng) BCA蛋白定量試劑盒
    別    名 BCA Kit; bicinchoninic acid; BCA Assay Kit; Bicinchoninic Acid Kit for Protein Determination; BCA Protein Quantitative Kit;   BCA蛋白濃度測定試劑盒; BCA法蛋白質(zhì)含量測定; BCA蛋白質(zhì)定量試劑盒; BCA蛋白質(zhì)含量試劑盒; BCA蛋白質(zhì)含量檢測試劑盒; BCA蛋白定量試劑盒; BCA蛋白定量試劑盒; BCA試劑盒; 蛋白含量測定; BCA 蛋白定量分析試劑盒;
    Specific References  (35)     |     C05-02001 has been referenced in 35 publications.
    [IF=13.281] Qin Ouyang. et al. Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease. 2022 Feb 19  Other ;  Other.  
    [IF=10.107] Zhao Yin. et al. Discovery of Berberine that Targetedly Induces Autophagic Degradation of both BCR-ABL and BCR-ABL T315I through Recruiting LRSAM1 for Overcoming Imatinib Resistance. Clin Cancer Res. 2020 Aug;26(15):4040-4053  other ;  
    [IF=9.417] Wen Zhang. et al. Construction of an ornidazole/bFGF-loaded electrospun composite membrane with a core-shell structure for guided tissue regeneration. MATER DESIGN. 2022 Sep;221:110960  
    [IF=8.911] Yin Z et al. Discovery of berberine that targetedly induce autophagic degradation of both BCR-ABL and BCR-ABL T315I through recruiting LRSAM1 for overcoming imatinib-resistance. Clin Cancer Res. 2020 Feb 25.  Other ;  
    [IF=7.666] Weiheng Zhang. et al. Long-Term Treatment with Gadopentetic Acid or Gadodiamide Increases TRPC5 Expression and Decreases Adriamycin Nuclear Accumulation in Breast Cancer Cells. CELLS-BASEL. 2023 Jan;12(9):1304  
    [IF=7.31] Zitong Ma. et al. Epimedii Folium and Ligustri Lucidi Fructus Promote Osteoblastogenesis and Inhibit Osteoclastogenesis against Osteoporosis via Acting on Osteoblast-Osteoclast Communication. OXID MED CELL LONGEV. 2023 Jan 31;2023:7212642  
    [IF=7.035] Jialiang Wei. et al. Protective role of TLR9-induced macrophage/microglia phagocytosis after experimental intracerebral hemorrhage in mice. CNS NEUROSCI THER. 2022 Jul;:  
    [IF=6.706] Zefu Chen. et al. Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins. NUTRIENTS. 2022 Jan;14(24):5222  
    [IF=6.638] Wang Z et al. Light-activatable?dual?prodrug?polymer?nanoparticle?for?precise?synergistic?chemotherapy?guidedby?drug-mediated?computed?tomography?imaging. Acta Biomater.?2019 May 22.  Other ;  
    [IF=6.208] Haibo Yao. et al. Whole-Transcriptome Sequencing of Antler Tissue Reveals That circRNA2829 Regulates Chondrocyte Proliferation and Differentiation via the miR-4286-R+1/FOXO4 Axis. INT J MOL SCI. 2023 Jan;24(8):7204  
    [IF=5.738] Gao Xiaoqiang. et al. Regulation of Gamma-Aminobutyric Acid Transaminase Expression and Its Clinical Significance in Hepatocellular Carcinoma. FRONT ONCOL. 2022 Jun;0:2857  
    [IF=5.56] Wang Haoxia. et al. Influences of flavonoids from Sedum aizoon L. on biofilm formation of Pseudomonas fragi. APPL MICROBIOL BIOT. 2023 Apr;:1-11  
    [IF=5.455] Qin, Gaofeng. et al. Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro. Chin Med-Uk. 2021 Dec;16(1):1-19  WB ;  Mouse.  
    [IF=5.195] Zhangbi Wu. et al. Ginsenoside-Rg1 mitigates cardiac arrest-induced cognitive damage by modulating neuroinflammation and hippocampal plasticity. EUR J PHARMACOL. 2023 Jan;938:175431  
    [IF=4.962] Yingying Chen. et al. SUZ12 participates in the proliferation of PNH clones by regulating histone H3K27me3 levels. 2022 Jan 06  Other ;  Other.  
    [IF=4.547] Li Jie. et al. S100A9 plays a key role in Clostridium perfringens beta2 toxin-induced inflammatory damage in porcine IPEC-J2 intestinal epithelial cells. BMC GENOMICS. 2023 Dec;24(1):1-14  
    [IF=3.974] Wei H et al. Alveolar macrophage reaction to PM2. 5 of hazy day in vitro: Evaluation methods and mitochondrial screening to determine mechanisms of biological effect.Ecotoxicol Environ Saf. 2019 Jun 15;174:566-573.  Other ;  
    [IF=3.69] Zitong Ma. et al. Comprehensive evaluation of the combined extracts of Epimedii Folium and Ligustri Lucidi Fructus for PMOP in ovariectomized rats based on MLP-ANN methods. J Ethnopharmacol. 2021 Mar;268:113563  WB ;  
    [IF=3.654] Mengying Zhang. et al. Preparation of Black pepper (Piper nigrum L.) essential oil nanoparticles and its antitumor activity on triple negative breast cancer in vitro. J FOOD BIOCHEM. 2022 Sep;:e14406  Other ;  Other.  
    [IF=3.575] Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 Dec;:  WB ;  Human.  
    [IF=3.53] Adil Hassan et al. Anti-atherosclerotic effects of Lactobacillus plantarum ATCC 14917 in ApoE ?/? mice through modulation of proinflammatory cytokines and oxidative stress. Appl Microbiol Biotechnol. 2020 Jul;104(14):6337-6350.  WB ;  Mouse.  
    [IF=3.399] SiZhe Yu. et al. NT157 Inhibits HCC Migration via Downregulating the STAT3/Jab1 Signaling Pathway:. Technol Cancer Res T. 2021;():  WB ;  Human.  
    [IF=3.318] Songsong Kang. et al. Tandem mass tag-based proteomics analysis reveals the effects of Guri Gumu-13 pill on drug-induced liver injury. J CHROMATOGR B. 2022 Jun;:123353  
    [IF=3.241] Tang X et al. Evaluation of CaD on bone structure and bone metabolic changes in senile osteoporosis rats based on MLP-ANN methods. Food Funct. 2019 Nov 25.  Other ;  
    [IF=3.093] Guo Xiao. et al. Effects of Transcutaneous Auricular Vagus Nerve Stimulation on Peripheral and Central Tumor Necrosis Factor Alpha in Rats with Depression-Chronic Somatic Pain Comorbidity. Neural Plast. 2020;2020:8885729  
    [IF=3.061] Jie Li. et al. Decreased S100A9 expression alleviates Clostridium perfringens beta2 toxin-induced inflammatory injury in IPEC-J2 cells. PEERJ. 2023 Jan;11:e14722  
    [IF=2.959] Peng W et al.Expression of osteoprotegerin and receptor activator for the nuclear factor-κB ligand in XACB/LV-bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis.(2018) J. Cell. Biochem. Oct 18  WB ;  
    [IF=2.532] Jianchao Ren. et al. Ejaculatory Duct Obstruction Affects Seminal Vesicle Contractile Efficacy and Smooth Muscle Ultrastructure in a Rat Model. ANDROLOGIA. 2023;2023:5022466  
    [IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
    [IF=2.031] Yujing Wang. et al. SIRT1 promotes proliferation, migration, and invasion of cervical cancer cell and is upregulated by human papillomavirus 16 E7 oncoprotein. Gynecol Obstet Inves. ;:  WB ;  Human.  
    CAS
    理論分子量 kDa
    檢測分子量
    保存條件 Store at -20℃.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    產(chǎn)品介紹 簡(jiǎn)介
    蛋白質(zhì)定量是蛋白質(zhì)研究的基礎工作之一。BCA (bicinchoninic acid)是理想的蛋白質(zhì)定量方法;是當前比Lowry法更優(yōu)越的專(zhuān)用于檢測總蛋白質(zhì)含量的產(chǎn)品。該方法以快速靈敏、穩定可靠,對不同種類(lèi)蛋白質(zhì)檢測的變異系數非常小而倍受專(zhuān)業(yè)人士的青睞。BCA法測定蛋白濃度不受絕大部分樣品中的化學(xué)物質(zhì)的影響。在組織細胞裂解實(shí)驗中,常用濃度的去垢劑SDS,Triton X-100,Tween不影響檢測結果,但受螯合劑(EDTA,EGTA)、還原劑(DTT,巰基乙醇)和脂類(lèi)的影響。實(shí)驗中,若發(fā)現樣品稀釋液或裂解液本身背景值較高,可試用Bradford蛋白濃度測定試劑盒。該產(chǎn)品不受此影響,因此與之配合使用,可免除您實(shí)驗中的許多煩惱。
    基本原理:堿性條件下,蛋白將Cu2+還原為Cu+, Cu+與BCA試劑形成紫顏色的絡(luò )合物,測定其在562nm處的吸收值,并與標準曲線(xiàn)對比,即可計算待測蛋白的濃度。
    BCA試劑的蛋白質(zhì)測定范圍是10-2000ug/ml

    使用說(shuō)明:
    1. 配制工作液: 根據標準品和樣品數量,按體積比A :B (50:1)配制適量BCA工作液,充分混勻。BCA工作液室溫24小時(shí)內穩定。
    2. 稀釋標準品:取10微升標準品用PBS稀釋至100ul(標準品一般可用PBS稀釋?zhuān)?,使終濃度為0.5mg/ml。將標準品按0, 1, 2, 4, 8, 12, 16, 20ul加到96孔板的蛋白標準品孔中,加PBS補足到20ul。
    3. 加適當體積樣品到96孔板的樣品孔中,補加PBS到20ul。
    4. 各孔加入200ul BCA工作液,37℃放置30分鐘。
    5. 冷卻到室溫,用酶標儀562nm測定OD值,根據標準曲線(xiàn)計算出蛋白濃度。

    注意事項:
    1. 在低溫條件或長(cháng)期保存出現沉淀時(shí),可攪拌或37℃溫育使溶解, 如發(fā)現細菌污染則應丟棄。
    2. 樣品中若含有EDTA、EGTA、DTT、硫酸銨、脂類(lèi)會(huì )影響檢測結果,請試用Bradford蛋白濃度測定試劑盒;高濃度的去垢劑也影響實(shí)驗結果,可用TCA沉淀去除干擾物質(zhì)。
    3.要得到更為精確的蛋白濃度結果,每個(gè)蛋白梯度和樣品均需做復孔, 每次均應做標準曲線(xiàn)。
    4.當試劑A和B混合時(shí)可能會(huì )有渾濁,但混勻后就會(huì )消失,工作液在密閉情況下可保存1周。
    5.需準備37℃水浴或溫箱、酶標儀或普通分光光度計,測定波長(cháng)為540-595nm之間,562nm最佳。酶標儀需與96孔酶標板配套使用。使用分光光度計測定蛋白濃度時(shí),試劑盒測定的樣品數量會(huì )因此而減 少。使用溫箱孵育時(shí),應注意防止因水粉蒸發(fā)影響檢測結果。


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