| 產(chǎn)品編號 | bsm-33198M |
| 英文名稱(chēng) | CBARA1/MICU1 Mouse mAb |
| 中文名稱(chēng) | 線(xiàn)粒體鈣吸收蛋白單克隆抗體 |
| 別 名 | Mitochondrial Calcium Uptake 1; Calcium-Binding Atopy-Related Autoantigen 1; Atopy-Related Autoantigen CALC; Ara CALC; CBARA1; CALC; Calcium Binding Atopy-Related Autoantigen 1; Calcium Uptake Protein 1, Mitochondrial; Allergen Hom S 4; EFHA3; MPXPS; MICU1_HUMAN; |
| 研究領(lǐng)域 | 細胞生物 信號轉導 新陳代謝 線(xiàn)粒體 |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Monoclonal |
| 克 隆 號 | 1H8 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 線(xiàn)粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CBARA1 Protein (1-250/476) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2013] Function: Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains (PubMed:20693986, PubMed:23101630, PubMed:23747253, PubMed:24313810, PubMed:24332854, PubMed:24503055, PubMed:24560927, PubMed:26341627, PubMed:26903221, PubMed:27099988). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake (PubMed:26903221). Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening (PubMed:26903221). Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm (PubMed:24560927, PubMed:26903221). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22904319). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (PubMed:16002733). Subunit: Homohexamer; in absence of calcium (PubMed:24514027). Forms a homohexamer in absence of calcium and rearranges into a heterodimer in presence of calcium (PubMed:26489515, PubMed:24514027). Heterodimer; disulfide-linked; heterodimerizes with MICU2 (PubMed:24560927). The heterodimer formed with MICU2 associates with MCU at low calcium concentration and dissociates from MCU at high calcium level (PubMed:26387864). Component of the uniplex complex, composed of MCU, MCUB, MICU1, MICU2 and EMRE/SMDT1 (PubMed:24231807). Interacts (via polybasic region) with EMRE/SMDT1; the interaction is direct (PubMed:27099988). Interacts (via polybasic region) with MCU (via coiled coil domains); the interaction is direct and precedes formation of the heterodimer with MICU2 (PubMed:21685886, PubMed:23101630, PubMed:23178883, PubMed:24332854, PubMed:26387864). Interacts with SLC25A23 (PubMed:24430870). Interacts with CHCHD4/MIA40; which introduces the interchain disulfide bond with MICU2 (PubMed:26387864). Subcellular Location: Mitochondrion inner membrane; Single-pass membrane protein; Mitochondrion intermembrane space Tissue Specificity: Expressed in epithelial cell lines. Strongly expressed in epidermal keratinocytes and dermal endothelial cells. Similarity: Belongs to the MICU1 family. MICU1 subfamily. Contains 2 EF-hand domains. SWISS: Q9BPX6 Gene ID: 10367 Database links: Entrez Gene: 10367 Human Entrez Gene: 216001 Mouse Omim: 605084 Human SwissProt: Q9BPX6 Human SwissProt: Q8VCX5 Mouse Unigene: 524367 Human Unigene: 27199 Mouse Unigene: 214252 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: U937 (Human) Cell Lysate at 30 ug
Lane 3: U87MG (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti- CBARA1/MICU1 (bsm-33198M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 54/46 kD
Observed band size: 54/46 kD
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