| 產(chǎn)品編號 | bsm-33018M |
| 英文名稱(chēng) | Mouse Anti-Trx-Tag antibody |
| 中文名稱(chēng) | 硫氧還蛋白單克隆抗體 |
| 別 名 | Thioredoxin; ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; TRDX; TRX 1; TRX; TRX1; TXN; TXN; ATL derived factor; ATL-derived factor; THIO_HUMAN; zgc:92903; Trx-Tag. |
| 產(chǎn)品類(lèi)型 | 標簽抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉導 轉錄調節因子 |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Monoclonal |
| 克 隆 號 | 7G10 |
| 交叉反應 | Recombinant protein |
| 產(chǎn)品應用 | WB=1:1000-5000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Thioredoxin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Function: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Subunit: Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner. Subcellular Location: Nucleus. Cytoplasm. Secreted. Note=Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. Similarity: Belongs to the thioredoxin family. Contains 1 thioredoxin domain. SWISS: P10599 Gene ID: 7295 Database links: Entrez Gene: 7295 Human Entrez Gene: 22166 Mouse Omim: 187700 Human SwissProt: P10599 Human SwissProt: P10639 Mouse Unigene: 435136 Human Unigene: 260618 Mouse Unigene: 29777 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Trx-tagged fusion protein (32-186) (bs-49104P) Overexpression E.coli Lysate at 2 ug
Lane 2: Trx-tagged fusion protein (19-213) (bs-49106P) Overexpression E.coli Lysate at 2 ug
Lane 3: Trx-tagged fusion protein (20-152) (bs-49107P) Overexpression E.coli Lysate at 2 ug
Primary: Anti-Trx-Tag (bsm-33018M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 30-40 kD
Observed band size: 30-40 kD
Sample: Lane 1: Recombined Trx/His-tag porcine?GM-CSF protein (Aa18-144) at 2 ug Lane 2: Enzyme digested Trx-tag Primary: Anti-Trx-Tag (bsm-33018M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 33/12 kD Observed band size: 33/12 kD
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