| 產品編號 | bs-13607R |
| 英文名稱 | CATSPERB Rabbit pAb |
| 中文名稱 | 陽離子通道精子相關蛋白β亞基抗體 |
| 別 名 | C14orf161; Cation channel sperm-associated protein subunit beta; Cation channel, sperm-associated, beta; Catsper channel auxiliary subunit beta; CatSper(beta); CATSPERB; FLJ14298; CTSRB_HUMAN. |
| 研究領域 | 細胞生物 免疫學 發育生物學 干細胞 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Dog) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 127 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CATSPERB: 851-950/1116 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CatSpers are ion transport proteins located on the surface of sperm cells in the principal piece of the sperm tail. CatSpers are vital to sperm motility, fertilization and cAMP-mediated calcium influx in sperm. There are four CatSper proteins in mammalian sperm, namely CatSper (or CatSper1), CatSper2, CatSper3 and CatSper4. CatSper proteins are believed to assemble into a heterotetrameric complex, forming an alkalinization-activated Ca2+-selective channel. CatSper∫ is a multipass membrane protein that functions as an auxiliary protein of the CatSper channel. It contains a large extracellular domain and a number of N-glycosylation sites. CatSper∫ specifically interacts with CatSper1 and through this interaction plays an important role in the hyperactivated motility of sperm cells, a process that is required in the preparation of sperm for fertilization. Function: RelevanceCATSPERB is a component of a complex composed at least of CATSPER1, CATSPERB, CATSPERG and HSPA1B. CATSPERB contains at least 2 transmembrane domains and 8 N-glycosylation sites. CATSPERB is required for trafficking or formation of a stable channel complex. It is involved in sperm cell hyperactivation via its association with CATSPER1. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. There are 2 isoforms produced by alternative splicing. Subcellular Location: Membrane; Multi-pass membrane protein SWISS: Q9H7T0 Gene ID: 79820 Database links: Entrez Gene: 79820 Human Omim: 611169 Human SwissProt: Q9H7T0 Human Unigene: 131755 Human |
| 產品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- CATSPERB (bs-13607R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 127 kD
Observed band size: 127 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
