| 產(chǎn)品編號 | bs-12378R |
| 英文名稱(chēng) | UCMA Rabbit pAb |
| 中文名稱(chēng) | 軟骨基質(zhì)相關(guān)蛋白UCMA抗體 |
| 別 名 | C10orf49; Gla rich protein; Gla-rich protein; Grp; GRP; OTTMUSP00000011599; RP23-272I15.1; UCMA; Ucma-C; UCMA_HUMAN; Unique cartilage matrix associated protein; Unique cartilage matrix-associated protein C-terminal fragment; Upper zone of growth plate and cartilage matrix associated. |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學(xué) 干細胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UCMA: 21-120/138 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
UCMA is a 138 amino acid secreted protein that is highly expressed in resting chrondrocytes in developing long bones and is thought to function in the early phase of chrondrocyte differentiation. A furin-like protease processes UCMA into an N-terminal 37 amino acid peptide and a C-terminal 74 amino acid peptide, which is referred to as Unique cartilage matrix-associated protein C-terminal fragment (Ucma-C). Introduction of recombinant Ucma-C interferes with osteogenic differentiation of mesenchymal stem cells, MC3T3-E1 preosteoblasts and primary osteoblasts. This suggests that Ucma may be involved in the negative regulation of osteogenic differentiation of osteochondrogenic precursor cells at the cartilage-bone interface and in peripheral zones of fetal cartilage. Function: May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface. Subcellular Location: Secreted > extracellular space > extracellular matrix. Tissue Specificity: Predominantly expressed in resting chondrocytes. Post-translational modifications: Proteolytically cleaved by a furin-like convertase to generate a persistent C-terminal fragment found in almost the entire cartilage matrix, and affecting osteoblast differentiation. Sulfated on tyrosine residues. Similarity: Belongs to the UCMA family. SWISS: Q8WVF2 Gene ID: 221044 Database links: Entrez Gene: 221044 Human SwissProt: Q8WVF2 human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (young rat paw); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCMA) Polyclonal Antibody, Unconjugated (bs-12378R) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining.
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