| 產(chǎn)品編號 | bs-12371R |
| 英文名稱(chēng) | Kindlin 2 Rabbit pAb |
| 中文名稱(chēng) | 絲裂原誘導蛋白2抗體 |
| 別 名 | FLJ34213; FLJ44462; UNC112; FERM2_HUMAN; Fermitin family homolog 2; Fermt2; KIND2; Kindlin-2; MIG-2; MIG2; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; Pleckstrin homology domain containing family C(with FERM domain) member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; UNC112B. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 發(fā)育生物學(xué) 信號轉導 干細胞 細胞粘附分子 細胞骨架 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Kindlin 2/MIG2: 352-420/680 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mig-2 recruits migfilin to cell-matrix adhesions, while the interaction with filamin mediates the association of migfilin with actin filaments. Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation. Mig-2 expression is transcriptionally elevated in leiomyomas and could be involved in its hormone-mediated growth of leiomyomas of the uterus. Expression of Mig-2 is ubiquitous, and it is found in numerous tumor tissues. Function: Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Subunit: Interacts with FBLP1. Subcellular Location: Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Note=Within actin stress fibers at cell-ECM focal adhesion sites. Tissue Specificity: Ubiquitous. Found in numerous tumor tissues. Similarity: Belongs to the kindlin family. Contains 1 FERM domain. Contains 1 PH domain. SWISS: Q96AC1 Gene ID: 10979 Database links: Entrez Gene: 10979 Human Entrez Gene: 218952 Mouse Omim: 607746 Human SwissProt: Q96AC1 Human SwissProt: Q8CIB5 Mouse Unigene: 509343 Human Unigene: 210018 Mouse Unigene: 7658 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kindlin 2) Polyclonal Antibody, Unconjugated (bs-12371R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kindlin 2) Polyclonal Antibody, Unconjugated (bs-12371R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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