| 產(chǎn)品編號 | bs-12270R |
| 英文名稱(chēng) | JLP/MAX binding protein Rabbit pAb |
| 中文名稱(chēng) | JNK相互作用蛋白4抗體 |
| 別 名 | C jun amino terminal kinase interacting protein 4; c Jun NH2 terminal kinase associated leucine zipper protein; FLJ13450; FLJ14006; FLJ34602; HLC 4; HLC 4 protein; HLC 6; HLC4; HLC4 protein; HLC6; HSS; Human lung cancer protein 6; Human lung cancer protein 6; JIP 4; JIP 4; JIP4; JNK associated leucine zipper protein 1; JNK associated leucine zipper protein; JNK interacting protein 4; JNK interacting protein 4; JNK interacting protein; JNK/SAPK associated protein; KIAA0516; Lung cancer oncogene 4; MAPK8IP4; Max binding protein; MGC117291; MGC14967; MGC74461; Mitogen activated protein kinase 8 interacting protein 4; Mitogen activated protein kinase 8 interacting protein 4; PHET; PIG 6; PIG6; Proliferation inducing gene 6; Protein highly expressed in testis; SPAG 9; SPAG9; Sperm associated antigen 9; Sperm specific protein; Sperm surface protein; Sunday driver(drosophila) homolog; Sunday driver 1; SYD1; JIP4_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學(xué) 干細胞 激酶和磷酸酶 表觀(guān)遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Sheep,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 146 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human JLP/JIP-4: 101-188/1321 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
JIP-4 is a 1,321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state. Function: JLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Subunit: Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1. Subcellular Location: Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. Tissue Specificity: Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients. Post-translational modifications: Phosphorylated by MAPK8 and MAPK14 (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the JIP scaffold family. SWISS: O60271 Gene ID: 9043 Database links: Entrez Gene: 9043 Human Entrez Gene: 70834 Mouse Omim: 605430 Human SwissProt: O60271 Human SwissProt: Q58A65 Mouse Unigene: 500367 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JLP MAX binding protein) Polyclonal Antibody, Unconjugated (bs-12270R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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