| 產(chǎn)品編號 | bs-7112R |
| 英文名稱(chēng) | EDG1 Rabbit pAb |
| 中文名稱(chēng) | 內皮細胞分化鞘脂G蛋白偶聯(lián)受體1抗體 |
| 別 名 | CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG 1; Endothelial differentiation G-protein coupled receptor 1; Endothelial differentiation sphingolipid G protein coupled receptor 1; G protein coupled sphingolipid receptor; S1P receptor 1; S1P receptor Edg-1; S1P1; |
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Specific References (3) | bs-7112R has been referenced in 3 publications.
[IF=6.117] Arianna Mazzoli. et al. SKELETAL MUSCLE INSULIN RESISTANCE AND ADIPOSE TISSUE HYPERTROPHY PERSIST BEYOND THE RESHAPING OF GUT MICROBIOTA IN YOUNG RATS FED A FRUCTOSE-RICH DIET. J NUTR BIOCHEM. 2022 Dec;:109247 WB ; Rat.
[IF=3.388] Xiao et al. Fingolimod Suppresses a Cascade of Core Vicious Cycle in Dry Eye NOD Mouse Model: Involvement of Sphingosine-1-Phosphate Receptors in Infiltrating Leukocytes. (2017) Invest.Ophthalmol.Vis.Sci. 58:6123-6132 IHC ; Mouse.
[IF=2.336] Gu X et al. SPNS 2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway. Clin Exp Pharmacol Physiol. 2019 Jun 17. WB ; Human.
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| 研究領(lǐng)域 | 細胞生物 信號轉導 細胞凋亡 細胞粘附分子 G蛋白偶聯(lián)受體 內皮細胞 細胞骨架 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EDG1/CD363: 51-150/382 <Extracellular> |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking. Function: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Post-translational modifications: S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P21453 Gene ID: 1901 Database links: Entrez Gene: 1901 Human Entrez Gene: 13609 Mouse SwissProt: P21453 Human SwissProt: O08530 Mouse 研究發(fā)現，S1P1與VEGF肩并肩相互協(xié)作，從而促進(jìn)血管生長(cháng)。VEGF是很多不同抗癌藥物的作用靶標。血管新生在很多疾病是異常的。靶向S1P1和VEGF可能要比只使用VEGF抑制劑更加有效地治療疾??；S1P1是一種關(guān)鍵性的血管新生反應性基因。研究人員證實(shí)當新的血管網(wǎng)絡(luò )形成時(shí)，由此產(chǎn)生的血液流動(dòng)激活血管內皮細胞表面上的S1P1，并把信號傳遞到這些細胞內部從而讓新形成的血管網(wǎng)絡(luò )穩定化。因此阻斷S1P1可能有助于切斷給癌性腫瘤提供資源的血液供應。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-EDG1 (bs-7112R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti-EDG1 (bs-7112R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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