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    HEC1 Rabbit pAb (bs-5727R)  
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    產(chǎn)品編號 bs-5727R
    英文名稱(chēng) HEC1 Rabbit pAb
    中文名稱(chēng) 癌細胞高表達蛋白Hec1抗體
    別    名 HEC 1; HEC; Highly Expressed in Cancer; Highly expressed in cancer protein; Highly expressed in cancer rich in leucine heptad repeats; HsHec1; hsNDC80; Kinetochore Associated 2; Kinetochore associated protein 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC 2; KNTC2; NDC 80; NDC80; NDC80_HUMAN; Retinoblastoma associated protein HEC; Retinoblastoma-associated protein HEC.  
    研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  細胞周期蛋白  
    抗體來(lái)源 Rabbit
    克隆類(lèi)型 Polyclonal
    克 隆 號
    交叉反應 Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse)
    產(chǎn)品應用 WB=1:500-2000
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 74 kDa
    檢測分子量
    細胞定位 細胞核 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human HEC1: 21-120/642 
    亞    型 IgG
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產(chǎn)品介紹 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

    Function:
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

    Subunit:
    Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis.

    Subcellular Location:
    Nucleus. Chromosome, centromere, kinetochore.

    Post-translational modifications:
    Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.

    Similarity:
    Belongs to the NDC80/HEC1 family.

    SWISS:
    O14777

    Gene ID:
    10403

    Database links:

    Entrez Gene: 10403 Human

    Entrez Gene: 67052 Mouse

    Omim: 607272 Human

    SwissProt: O14777 Human

    SwissProt: Q9D0F1 Mouse

    Unigene: 414407 Human

    Unigene: 225956 Mouse



    產(chǎn)品圖片
    Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- HEC1 (bs-5727R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 83 kD
    Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- HEC1 (bs-5727R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 74 kD
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