| 產(chǎn)品編號 | bs-2811R |
| 英文名稱(chēng) | HDAC6 Rabbit pAb |
| 中文名稱(chēng) | 組蛋白去乙?；?抗體 |
| 別 名 | HD 6; HD6; HDAC 6; Histone deacetylase 6; HD6; Histone deacetylase 6; JM 21; JM21; KIAA0901; FLJ16239; HDAC6_HUMAN. |
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Specific References (1) | bs-2811R has been referenced in 1 publications.
[IF=6.684] Huihua Kai. et al. LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6. Front Cell Dev Biol. 2021; 9: 701628 WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 發(fā)育生物學(xué) 信號轉導 細胞凋亡 轉錄調節因子 表觀(guān)遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 134 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HDAC6: 301-400/1215 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]. Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy. Subunit: Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15 (By similarity). Interacts with DDIT3/CHOP. Subcellular Location: Nucleus. Cytoplasm. Note=It is mainly cytoplasmic, where it is associated with microtubules. Post-translational modifications: Phosphorylated by AURKA. Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger. SWISS: Q9UBN7 Gene ID: 10013 Database links: Entrez Gene: 10013 Human Entrez Gene: 15185 Mouse Omim: 300272 Human SwissProt: Q9UBN7 Human SwissProt: Q9Z2V5 Mouse Unigene: 6764 Human Unigene: 29854 Mouse Unigene: 13453 Rat ?組蛋白去乙?；?HDACs)是一組在細胞染色質(zhì)水平、通過(guò)誘導組蛋白去乙?；瘉?lái)調控包括染色質(zhì)重組、轉錄活化或抑制、細胞周期、細胞分化及細胞凋亡等一系列生物學(xué)效應的酶，特別是與細胞活化后的基因轉錄表達調控有關(guān)。 ??? HDAC6是一種比較獨特的組蛋白去乙?；?，含有兩個(gè)功能上相互獨立的HDAC催化結構域。HDAC6可以去乙?；M蛋白并抑制相關(guān)基因轉錄。 ????HDAC6可以和微管(microtuble)結合，可以去乙?；痶ubulin，Hsp90和cortactin等。目前發(fā)現大量的蛋白可以被乙?；揎?，因此HDAC等乙?；揎椕副徽J為在基因轉錄調控、信號轉導、生長(cháng)發(fā)育、分化凋亡、代謝性疾病和腫瘤等多種生理病理過(guò)程中發(fā)揮重要作用。HDAC的抑制劑目前被認為是很有前景的腫瘤治療藥物。 ????內源性HDAC6主要定位于細胞漿，與微管相結合并且是一個(gè)微管蛋白去乙?；?。HDAC6含有一個(gè)鋅指結構域，該結構域可能和泛素化降解的調節有關(guān)。HDAC6可以和DHAC11相互作用。 |
| 產(chǎn)品圖片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-HDAC6 (bs-2811R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 134 kD
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDAC6) Polyclonal Antibody, Unconjugated (bs-2811R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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