| 產(chǎn)品編號 | bs-1926R |
| 英文名稱(chēng) | UCP-2 Rabbit pAb |
| 中文名稱(chēng) | 線(xiàn)粒體脫偶連蛋白2抗體 |
| 別 名 | Uncoupling Protein-2; UCP2; UCP 2; BMIQ4; Mitochondrial uncoupling protein 2; SLC25A8; UCPH; Uncoupling protein 2; Uncoupling protein 2 mitochondrial proton carrier; Solute carrier family 25 member 8; UCP2_MOUSE. |
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Specific References (6) | bs-1926R has been referenced in 6 publications.
[IF=5.988] Lei Zhao. et al. Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities. FRONT PHARMACOL. 2022; 13: 933669 WB ; Mouse.
[IF=5.469] Tidwell, Tia R.. et al. Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines. CANCER METAB. 2022 Dec;10(1):1-16 FC ; Human.
[IF=4.35] Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227 WB ; Mouse.
[IF=4.35] Chuang, Yao-Chung, et al. "Peroxisome proliferator activated receptors γ/mitochondrial uncoupling protein 2 signaling protects against seizure-induced neuronal cell death in the hippocampus following experimental status Rat.
[IF=2.552] Gaochun Zhu . et al. Mitochondrial uncoupling protein 2 is regulated through heterogeneous nuclear ribonucleoprotein K in lead exposure models. J Environ Sci Heal C. 2021;39(1):1-16 WB ; Rat.
[IF=2.47] Bo, Jinshuang, et al. "Methylglyoxal Impairs Insulin Secretion of Pancreatic β-Cells through Increased Production of ROS and Mitochondrial Dysfunction Mediated by Upregulation of UCP2 and MAPKs." Journal of Diabetes Research(2015). WB ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 染色質(zhì)和核信號 糖尿病 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse UCP-2: 201-309/309 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes. Function: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Subunit: Acts as a dimer forming a proton channel (By similarity). Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. Database links: Entrez Gene: 7351 Human Omim: 601693 Human SwissProt: P55851 Human Unigene: 80658 Human Unigene: 13333 Rat UCP-2又稱(chēng)解耦聯(lián)蛋白 2 (UCP2 )是 1997年發(fā)現的一種新的解偶聯(lián)蛋白 ,它是線(xiàn)粒體內膜載體家族的一員 。與其類(lèi)似物UCP1相比 ,UCP2在棕色脂肪組織中含量較低 ,但在體內分布廣泛。解耦聯(lián)蛋白(UCP)具有使線(xiàn)粒體呼吸鏈解耦聯(lián)作用,解耦聯(lián)所釋放的能量以熱能的形式散發(fā)。在對UCP與產(chǎn)熱關(guān)系的研究中,發(fā)現UCP2具有調節機體能量代謝的功能,并且可能是肥胖與2型糖尿病相關(guān)聯(lián)的一個(gè)重要基因。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Stomach (Rat) Lysate at 40 ug
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 3: SiHa (Human) Cell Lysate at 30 ug
Lane 4: DU145 (Human) Cell Lysate at 30 ug
Lane 5: RAW264.7 (Mouse) Cell Lysate at 30 ug
Lane 6: Liver (Mouse) Lysate at 40 ug
Primary: Anti-UCP-2 (bs-1926R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCP-2) Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (bs-1926R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (bs-1926R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-UCP-2 antibody (bs-1926R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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