| 產(chǎn)品編號 | bs-0979R |
| 英文名稱(chēng) | CD38 Rabbit pAb |
| 中文名稱(chēng) | CD38抗體 |
| 別 名 | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrola |
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Specific References (3) | bs-0979R has been referenced in 3 publications.
[IF=14.919] Jinfeng Wang. et al. Translocation of vaginal microbiota is involved in impairment and protection of uterine health. Nat Commun. 2021 Jul;12(1):1-15 IF ; Rat.
[IF=4.966] Santoshi Muppalaet al. The P387 thrombospondin‐4 variant promotes accumulation of macrophages in atherosclerotic lesions. FASEB J
. 2020 Sep;34(9):11529-11545. IHC, IF ; mouse.
[IF=1.257] Moridera K et al. Skeletal unloading reduces cluster of differentiation (CD) 38 expression in the bone marrow and osteoblasts of mice. J Orthop Sci.?2019 May 6. ICC ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 干細胞 糖尿病 細胞表面分子 自然殺傷細胞 淋巴細胞 t-淋巴細胞 b-淋巴細胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | 45 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD38: 101-200/304 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Function: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. Similarity: Belongs to the ADP-ribosyl cyclase family. Database links: Entrez Gene: 952 Human Entrez Gene: 12494 Mouse Omim: 107270 Human SwissProt: P28907 Human SwissProt: P56528 Mouse Unigene: 479214 Human Unigene: 249873 Mouse CD38分子是單鏈Ⅱ型跨膜糖蛋白，存在于很多免疫細胞表面上,CD38與無(wú)脊椎動(dòng)物卵的授精、HIV感染、某些癌癥以及糖尿病等有關(guān)，CD38具有許多復雜而又獨特的生物學(xué)特性及功能，有調控細胞鈣釋放的機制。主要表達于胸腺細胞、前T和前B細胞、活化的T和B細胞、單核細胞、NK細胞、漿細胞等，可用于急性白血病的分型和活化的T細胞在自身免疫和免疫缺陷中的作用等方面的研究，也可作為生發(fā)中心B細胞的一種選擇性標記。 |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 48 kD
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 48 kD
Sample:
SP2/0(Mouse) Cell Lysate at 30 ug
Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 54 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD38 Polyclonal Antibody, Unconjugated(bs-0979R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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