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    CCNA2 Rabbit pAb (bs-0571R)  
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    產(chǎn)品編號 bs-0571R
    英文名稱(chēng) CCNA2 Rabbit pAb
    中文名稱(chēng) 周期素A2抗體
    別    名 CCNA2_HUMAN; Cyclin-A2; Cyclin-A; CCN1; CCN1; CCNA; cyclin A2;  
    Specific References  (4)     |     bs-0571R has been referenced in 4 publications.
    [IF=8.74] Chen et al. STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7. (2015) Cancer.Cell.Int. 15:111  WB ;  Human.  
    [IF=5.31] Meiju Zhang. et al. The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells. J Cell Mol Med. 2022 Feb;26(4):1128-1143  IHC ;  Mouse.  
    [IF=2.41] Chang et al. Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level. (2015) BMC.Cell.Biol. 16:25  WB ;  Mouse.  
    [IF=2.08] Zhang, Jihong, et al. "Interleukin 18 augments growth ability via NF-κB and p38/ATF2 pathways by targeting cylin B1, cyclin B2, cylin A2, and Bcl-2 in BRL-3A rat liver cells." Gene (2015).  WB ;  Rat.  
    研究領(lǐng)域 腫瘤  心血管  細胞生物  細胞周期蛋白  
    抗體來(lái)源 Rabbit
    克隆類(lèi)型 Polyclonal
    克 隆 號
    交叉反應 Human,Mouse (predicted: Rat)
    產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 49 kDa
    檢測分子量
    細胞定位 細胞漿 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CCNA2: 375-431/431 
    亞    型 IgG
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產(chǎn)品介紹 The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]

    Function:
    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.

    Subunit:
    Interacts with the CDK1 and CDK2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. When associated with CDK2 (but not with CDK1), interacts with SCAPER.

    Subcellular Location:
    Nucleus. Cytoplasm. Note=Cytoplasmic when associated with SCAPER.

    Post-translational modifications:
    Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

    Similarity:
    Belongs to the cyclin family. Cyclin AB subfamily.

    SWISS:
    P20248

    Gene ID:
    890

    Database links:

    Entrez Gene: 281667 Cow

    Entrez Gene: 890 Human

    Entrez Gene: 12428 Mouse

    Entrez Gene: 114494 Rat

    Omim: 123835 Human

    SwissProt: P30274 Cow

    SwissProt: P20248 Human

    SwissProt: P51943 Mouse

    Unigene: 58974 Human

    Unigene: 4189 Mouse

    Unigene: 13094 Rat



    Cyclin A 可以出現在所有類(lèi)型的細胞。各周期素可出現在不同的細胞增殖周期中。能結合和激活CDK2激酶,促進(jìn)細胞G1/S和G2/M增殖周期的轉化。
    產(chǎn)品圖片
    Tissue/cell: human bile duct cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin A2 Polyclonal Antibody, Unconjugated(bs-0571R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin A2 Polyclonal Antibody, Unconjugated(bs-0571R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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